Vitrification of immature oocytes of goats in Bangladesh
نویسندگان
چکیده
منابع مشابه
I-14: Vitrification of Human Immature Oocytes in IVM Program
The cryopreservation of immature GV oocytes by vitrification is offering attractive perspectives, since they represent an important pool of germ cells for women undergoing IVM cycles, or at risk of infertility for OHSS or PCOs, particularly those poor responders to gonadotropin stimulation, or in case of cancer. Therefore, it becomes necessary to optimize both cryopreservation technique as well...
متن کاملUltrastructure of immature and mature human oocytes after cryotop vitrification
In vitro maturation of vitrified immature germinal vesicle (GV) oocytes is a promising fertility preservation option. We analyzed the ultrastructure of human GV oocytes after Cryotop vitrification (GVv) and compared it with fresh GV (GVc), fresh mature metaphase II (MIIc) and Cryotop-vitrified mature (MIIv) oocytes. By phase contrast microscopy and light microscopy, the oolemmal and cytoplasmic...
متن کاملVitrification of immature feline oocytes with a commercial kit for bovine embryo vitrification.
The aim of this study was to evaluate the suitability of a commercial kit for bovine embryo vitrification for cryopreserving cat oocytes and to evaluate comparatively the effects of its use with slow freezing procedure on cryotolerance in terms of morphology and oocyte resumption of meiosis. Germinal vesicle stage oocytes isolated from cat ovaries were either vitrified (n = 72) using a vitrific...
متن کاملVitrification Affects Nuclear Maturation and Gene Expression of Immature Human Oocytes
Background: Vitrification of oocytes is a fast-freezing technique, which may affect the quality of the human oocyte, and consequently affects the embryo development, pregnancy and birth. The aim of the current study was to investigate the consequence of in-vitro vitrification on maturation status of immature human oocytes, additionally, expression levels of stress, and apoptosis related genes. ...
متن کاملVitrification of immature bovine oocytes using glass micropipettes under normal or super-cooled LN Vitrification and thawing: Two vitrification solutions
Aim: To evaluate the developmental competence of post-thaw vitrified bovine cumulus-oocyte complexes (COCs) in vitro. Materials and Methods: A total of 129 COCs were cryopreserved using vitrification solution comprising of 15% ethylene glycol (EG) + 15% dimethyl sulfoxide (DMSO) + 0.6 M sucrose in medium TCM-199 with 10% FBS. Immediately, within a minute they are plunged into liquid nitrogen us...
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ژورنال
عنوان ژورنال: Bangladesh Veterinarian
سال: 2019
ISSN: 2224-7289,1012-5949
DOI: 10.3329/bvet.v35i1-2.53382